Epidemiological evaluation ofNeisseria meningitidisserogroup B by pulsed-field gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Pulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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Hiroko WAKIMOTO1)2), Masamichi OGURA2), Hisako YANO2), Akira KOGUCHI3), Yukio WAKIMOTO4), Souhei MIYAKAWA5), Matsuko Katsuko OKUZUMI6) & Masashi MIZOKAMI4) 1) Aichi Medical University School of Nursing 2) Nagoya City University Graduate School of Nursing 3) Abbott Japan 4) Nagoya City University Hospital 5) Kariwa-gun General Hospital 6) Dokkyo Medical University Hospital (Received November 2, ...
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A versatile system (PHOGE) has been developed that allows resolution of molecules of DNA megabase pair size by the use of homogeneous, orthogonal, pulsed fields. The resulting electrophoretograms have characteristics that differ from those produced by other systems for pulsed field electrophoresis. Molecules in a two-fold range of sizes can be separated with maximum resolution, or a much larger...
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Thal et al. (4) have recently reported data comparing the macro-restriction fragment patterns of clonal Enterococcus faecalis isolates differing in the chromosomal location of the conjugative transposon Tn916. They observed, in relation to the parental strain FA2-2, one to two restriction fragment differences resulting from a single Tn916 insertion and two to four differences associated with tw...
متن کاملPulsed-field gel electrophoresis of circular DNA.
Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 1995
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.1995.tb00032.x